Top seed Svetlana Kuznetsova was beaten 6-2, 6-2 by Denmark's Caroline Wozniacki in the second round of the Eastbourne International on Wednesday.
The grasscourt tournament was also hit by the withdrawal of former Wimbledon champion Amelie Mauresmo who retired in her match against Australia's Samantha Stosur with a thigh strain.
"I just felt something on the last point of my first service game and then, during the returning game, I felt that I couldn't really move that well any more or push off my left leg," Mauresmo, the 2006 Wimbledon champion, told reporters.
"With this happening at the beginning of the match, I didn't feel like taking any risks before Wimbledon next week."
Third seed Vera Zvonareva had her Wimbledon preparations cut short when she lost to fellow Russian Ekaterina Makarova 6-3, 6-3, who set up another all-Russian clash against Nadia Petrova.
There was better news for second seed Marion Bartoli as last year's surprise runner-up at Wimbledon made short work of Austrian Sybille Bammer, dropping just three games to reach the quarter-final.
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